Disulfide Bond Formation in Bacterial Cell-Free Systems
Cell-free systems need multiple layers to form disulfide bonds correctly.
Liesel Brandhorst
Biology Unbound — Page 3
Protein Expression
Membrane Protein Expression in Cell-Free Systems
A technique that eliminates living cells finally cracks how to produce functional membrane proteins.
Inclusion Body-Prone Protein Solubility in CFPS
Cell-free synthesis solves protein aggregation by removing the constraints that living cells impose.
Tomás Reyes-Fuentes
More From Biology Unbound
Wheat Germ and Rabbit Reticulocyte Systems vs. Bacterial CFPS
Bacterial extracts work for most proteins; pick eukaryotic systems only when folding demands it.
Tomás Reyes-Fuentes
Lot-to-Lot Variability in Cell-Free Reagent Kits
Standardized reagent prep, not extract quality, controls variability across labs.
Tomás Reyes-Fuentes
DNA Template Design for Maximizing CFPS Yield
Optimize DNA templates before blaming reagents for weak cell-free protein synthesis.
Priya Subramaniam
Cell-Free vs. Cell-Based Protein Expression Decision Framework
A structured framework helps scientists pick the right protein expression system every time.
Tomás Reyes-Fuentes
Magnesium and Potassium Optimization in CFPS Reactions
Free magnesium matters more than total magnesium in protein synthesis reactions.
Marcus Oyelaran
Energy Regeneration Systems in Cell-Free Protein Synthesis
ATP depletion and phosphate buildup sabotage cell-free protein synthesis.
Marcus Oyelaran
Coupled vs. Uncoupled Transcription-Translation in CFPS
Coupling transcription and translation boosts protein yield by loading ribosomes before mRNA folds.
Marcus Oyelaran
E. coli Extract Preparation for Cell-Free Systems
Each prep stage protects active machinery from the degradative enzymes riding along in the extract.
Priya Subramaniam
Unnatural Amino Acid Incorporation via Cell-Free Suppression
Cell-free synthesis eliminates three barriers to unnatural amino acid incorporation simultaneously.
Priya Subramaniam