Production WorkflowsIsotopic Labeling of Proteins for NMR Using Cell-Free Systems
Cell-free protein synthesis enables precise isotope labeling without metabolic interference.
Liesel Brandhorst·
Production WorkflowsProtein Purification Strategies Downstream of CFPS Reactions
CFPS platform choice determines purification difficulty and cost before the reaction even starts.
Liesel Brandhorst·
Production WorkflowsCost per Milligram Benchmarks for AI-Designed Antibody Campaigns Across Scale
Cell-free synthesis closes the gap between AI's design speed and experimental validation capacity.
Liesel Brandhorst·
Production WorkflowsReaction Condition Scaling From Microwell to Milliliter CFPS Formats
Liesel Brandhorst·
Emerging CFPS ApplicationsCell-Free Protein Synthesis for Neoantigen and Research Antigen Production
A faster, simpler way to make hard-to-express research antigens.
Liesel Brandhorst·
Emerging CFPS ApplicationsCell-Free Production of Difficult Targets for Structural Biology Sample Prep
Cell-free systems overcome toxicity and insolubility that sabotage conventional protein expression.
Liesel Brandhorst·
Variant ScreeningCFPS Reaction Miniaturization Below 10 µL
Liesel Brandhorst·
Protein ExpressionMembrane Protein Expression in Cell-Free Systems
A technique that eliminates living cells finally cracks how to produce functional membrane proteins.
Liesel Brandhorst·
Protein ExpressionDisulfide Bond Formation in Bacterial Cell-Free Systems
Cell-free systems need multiple layers to form disulfide bonds correctly.
Liesel Brandhorst·